DNI may stand for:

• Direct neural interface, a brain-computer interface
• Direct normal irradiance, a measure of the Solar irradiance striking a surface held normal to the line of sight to the sun
• Direct normal insolation, also known as direct insolation, a measure of the solar irradiance striking a surface held normal to line of sight to the sun
• Digital Network Intelligence, the NSA term for the collection of data from the Internet
• Director of National Intelligence, a Cabinet-level executive position that oversees the United States Intelligence Community
• Director of Naval Intelligence (disambiguation)
• Documento Nacional de Identidad (Spain), the Spanish national identity document
• Documento Nacional de Identidad (Argentina), the Argentine national identity document
• Dole Nutrition Institute founded by the Dole Food Company
• Do not intubate, an alternate term for "do not resuscitate"
• Do not install, a term sometimes used in Printed circuit board design to denote the omitting of a component
• Do not interact, a term used to deter people or a certain group of people from interacting with someone or something.
• Lower Grand Valley Dani language (ISO 639 code: dni)
• Wad Medani Airport (IATA airport code: DNI), an airstrip in Sudan